Abstract
UPLC-MS is a commonly used technique to first separate complex samples and subsequently quantify molecules of interest. Herein we describe the use of UPLC-MS using an amide stationary phase to quantify non-derivatized amino acids extracted from fingerprints. As detector either a triple-quadrupole MS/MS or a TOF-MS detector was used. This method allows for a simple and fast sample preparation, which facilitates the analysis of large amounts of samples.
Original language | English |
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Title of host publication | Amino acid analysis |
Subtitle of host publication | methods and protocols |
Editors | Michail A. Alterman |
Place of Publication | New York |
Publisher | Humana Press Inc. |
Chapter | 33 |
Pages | 439-450 |
Edition | 2 |
ISBN (Electronic) | 9781493996391 |
ISBN (Print) | 9781493996384 |
DOIs | |
Publication status | Published - 2019 |
Publication series
Name | Methods in molecular biology (Clifton, N.J.) |
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Volume | 2030 |
ISSN (Print) | 1064-3745 |
Funding
W. v. H. acknowledges a NWO research grant (no. 2014-01-124PRO).