Amino acid profiling from fingerprints: using Amide Stationary-Phase UPLC-MS

Ward van Helmond, Marcel de Puit

Research output: Chapter in Book/Report/Conference proceedingChapterAcademicpeer-review


UPLC-MS is a commonly used technique to first separate complex samples and subsequently quantify molecules of interest. Herein we describe the use of UPLC-MS using an amide stationary phase to quantify non-derivatized amino acids extracted from fingerprints. As detector either a triple-quadrupole MS/MS or a TOF-MS detector was used. This method allows for a simple and fast sample preparation, which facilitates the analysis of large amounts of samples.

Original languageEnglish
Title of host publicationAmino acid analysis
Subtitle of host publicationmethods and protocols
EditorsMichail A. Alterman
Place of PublicationNew York
PublisherHumana Press Inc.
ISBN (Electronic)9781493996391
ISBN (Print)9781493996384
Publication statusPublished - 2019

Publication series

NameMethods in molecular biology (Clifton, N.J.)
ISSN (Print)1064-3745


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