UPLC-MS is a commonly used technique to first separate complex samples and subsequently quantify molecules of interest. Herein we describe the use of UPLC-MS using an amide stationary phase to quantify non-derivatized amino acids extracted from fingerprints. As detector either a triple-quadrupole MS/MS or a TOF-MS detector was used. This method allows for a simple and fast sample preparation, which facilitates the analysis of large amounts of samples.
|Title of host publication||Amino acid analysis|
|Subtitle of host publication||methods and protocols|
|Editors||Michail A. Alterman|
|Place of Publication||New York|
|Publisher||Humana Press Inc.|
|Publication status||Published - 2019|
|Name||Methods in molecular biology (Clifton, N.J.)|