Amino acid profiling from fingerprints: using Amide Stationary-Phase UPLC-MS

Ward van Helmond; Marcel de Puit

doi:10.1007/978-1-4939-9639-1_33

Amino acid profiling from fingerprints: using Amide Stationary-Phase UPLC-MS

Ward van Helmond
, Marcel de Puit

Forensic Science

Research output: Chapter in Book/Report/Conference proceeding › Chapter › Academic › peer-review

Abstract

UPLC-MS is a commonly used technique to first separate complex samples and subsequently quantify molecules of interest. Herein we describe the use of UPLC-MS using an amide stationary phase to quantify non-derivatized amino acids extracted from fingerprints. As detector either a triple-quadrupole MS/MS or a TOF-MS detector was used. This method allows for a simple and fast sample preparation, which facilitates the analysis of large amounts of samples.

Original language	English
Title of host publication	Amino acid analysis
Subtitle of host publication	methods and protocols
Editors	Michail A. Alterman
Place of Publication	New York
Publisher	Humana Press Inc.
Chapter	33
Pages	439-450
Edition	2
ISBN (Electronic)	9781493996391
ISBN (Print)	9781493996384
DOIs	https://doi.org/10.1007/978-1-4939-9639-1_33
Publication status	Published - 2019

Publication series

Name	Methods in molecular biology (Clifton, N.J.)
Volume	2030
ISSN (Print)	1064-3745

Funding

W. v. H. acknowledges a NWO research grant (no. 2014-01-124PRO).

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abstract = "UPLC-MS is a commonly used technique to first separate complex samples and subsequently quantify molecules of interest. Herein we describe the use of UPLC-MS using an amide stationary phase to quantify non-derivatized amino acids extracted from fingerprints. As detector either a triple-quadrupole MS/MS or a TOF-MS detector was used. This method allows for a simple and fast sample preparation, which facilitates the analysis of large amounts of samples.",

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Amino acid profiling from fingerprints: using Amide Stationary-Phase UPLC-MS. / van Helmond, Ward; de Puit, Marcel.
Amino acid analysis: methods and protocols. ed. / Michail A. Alterman. 2. ed. New York: Humana Press Inc., 2019. p. 439-450 (Methods in molecular biology (Clifton, N.J.); Vol. 2030).

Research output: Chapter in Book/Report/Conference proceeding › Chapter › Academic › peer-review

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Amino acid profiling from fingerprints: using Amide Stationary-Phase UPLC-MS

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