Increased species richness in the oral microbiome by a 13-week protein and combined lifestyle intervention in older adults with obesity and type 2 diabetes

M. Haji, R.G. Memelink, B.W. Brandt, M.J. Buijs, W.J. Pasman, J. de Vogel-van den Bosch, S. Wopereis, E. Zaura, Peter Weijs

Research output: Contribution to journalMeeting AbstractAcademic

Abstract

Rationale: While combined lifestyle interventions have multiple health benefits, their impact on the oral microbiome is not
known. We explored the effects of a lifestyle intervention including protein drink on the oral microbiome in older adults
with obesity and type 2 diabetes (T2D).
Methods: In a post-hoc analysis of the PROBE study, 87 subjects (66.5±6.1 years, 33% female) with tongue dorsum
samples at baseline and week 13 were included. All subjects participated in a 13-week lifestyle intervention with exercise
(3x/week) and hypocaloric diet (-600 kcal/day), and had been randomized to receive a test product (21g whey protein
enriched with leucine and vitamin D) or isocaloric control (0g protein) 10x/week. T2D was subtyped as muscle insulin
resistance (MIR, n=34) or no-MIR (n=36) based on available muscle insulin sensitivity index. Microbiome was analysed
by V4 16s rDNA sequencing. Diversity, measured as species richness and Shannon diversity index, was statistically
analysed with paired (within group) and independent (between groups) samples t-test.
Results: In the MIR subgroup, species richness (baseline 73.1±19.1, wk13 88.1±24.9; p<0.001) and Shannon diversity
index (baseline 2.8±0.3, wk13 3.0±0.3; p=0.01) increased significantly over time, and change in species richness was
significantly different between test (+15.0±11.5) and control (+0.7±18.2) (p=0.01). On the whole group level, diversity did
not change over time and did not differ between study groups. Change in species richness significantly correlated with
change in BMI (r=-0.25, p=0.02) and change in fasting glucose (r=-0.28, p=0.01).
Conclusion: Consuming a whey protein drink enriched with leucine and vitamin D during a combined lifestyle intervention
increased species richness of the oral microbiome in obese T2D subjects with muscle insulin resistance.
Original languageEnglish
JournalClinical Nutrition ESPEN
Volume46
Publication statusPublished - 1 Dec 2021
EventESPEN virtual congress on Clinical Nutrition & Metabolism -
Duration: 9 Sep 202114 Sep 2021

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