Abstract
Semen traces are considered important pieces of evidence in forensic investigations, especially those involving sexsual offenses. Recently, our research group developed a fluorescence-based technique to accurately determine the age of semen traces. However, the specific compounds resonsible for the fluoresescent behaviour of ageing semens remain unknown. As such, in this exploratory study, the aim is to identify the components associated with the fluorescent behavior of ageing semen traces. In this investigation semen stains and various biofluorophores commonly found in body fluids were left to aged for 0, 2, 4, 7, 14 and 21 days. Subsequently, thin-layer chromatography (TLC) and ultra-performance liquid chromatography (UPLC) mass spectrometry were performed to identify the biofluorophores present in semen. Several contributors to the autofluorescence could be identified in semen stain, these include tryptophan, kynurenine, kynurenic acid, and norharman. The study sheds light on the.
| Original language | English |
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| Article number | 124278 |
| Pages (from-to) | 1-5 |
| Journal | Journal of Chromatography B |
| Volume | 1246 |
| DOIs | |
| Publication status | Published - 1 Oct 2024 |
Funding
This research is supported by the Dutch Technology Foundation STW, which is part of The Netherlands Organization for Scientific Research (NWO), and which is partly funded by the Ministry of Economic Affairs, Project number: 14430.
| Funders | Funder number |
|---|---|
| Nederlandse Organisatie voor Wetenschappelijk Onderzoek | Project number: 14430 |